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rat multiple tissue northern blot  (TaKaRa)


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    TaKaRa rat multiple tissue northern blot
    ( A ) <t>Northern</t> <t>blot</t> analysis of <t>rat</t> iap mRNA expression in adult rat tissues. A rat <t>multiple</t> <t>tissue</t> northern blot (Clontech) containing 2 μg/lane poly(A) + RNA per lane was probed sequentially with [ 32 P] dCTP (Amersham) labeled, random primed (Amersham Rediprime) DNA probes derived from the coding regions of rat iap-1 , iap-2 , iap-3, and β-actin (control). Blots were hybridized overnight in 5 × SSPE/10 × Denhardt's solution/100 μg/ml salmon sperm DNA/ 50% formamide/ 2% SDS and then washed with 0.2 × SSC/ 0.1% SDS at 50°C. The position and sizes of the markers are indicated on the left. The tissues represented on the blot are as follows: ( 1 ) heart, ( 2 ) brain, ( 3 ) spleen, ( 4 ) lung, ( 5 ) liver, ( 6 ) skeletal muscle, ( 7 ) kidney, and ( 8 ) testis. ( B ) Northern blot analysis of the expression of iap -2 in testes. A rat multiple tissue Northern blot (Clontech; same as in (A)) was hybridized sequentially with [ 32 P] dCTP labeled DNA probes specific to iap -2 coding region, BIR domain and the RING zing finger. The blots were processed as in (A). The position of testis-specific transcript is indicated by an asterisk ( * ).
    Rat Multiple Tissue Northern Blot, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+multiple+tissue+northern+blots/Rat+Brain+Poly+A%2B+RNA/pmc00065541-85-1-6
    Average 90 stars, based on 96 article reviews
    rat multiple tissue northern blot - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Cloning and characterization of the rat homologues of the Inhibitor of Apoptosis protein 1, 2, and 3 genes."

    Article Title: Cloning and characterization of the rat homologues of the Inhibitor of Apoptosis protein 1, 2, and 3 genes.

    Journal: BMC Genomics

    doi: 10.1186/1471-2164-3-5

    ( A ) Northern blot analysis of rat iap mRNA expression in adult rat tissues. A rat multiple tissue northern blot (Clontech) containing 2 μg/lane poly(A) + RNA per lane was probed sequentially with [ 32 P] dCTP (Amersham) labeled, random primed (Amersham Rediprime) DNA probes derived from the coding regions of rat iap-1 , iap-2 , iap-3, and β-actin (control). Blots were hybridized overnight in 5 × SSPE/10 × Denhardt's solution/100 μg/ml salmon sperm DNA/ 50% formamide/ 2% SDS and then washed with 0.2 × SSC/ 0.1% SDS at 50°C. The position and sizes of the markers are indicated on the left. The tissues represented on the blot are as follows: ( 1 ) heart, ( 2 ) brain, ( 3 ) spleen, ( 4 ) lung, ( 5 ) liver, ( 6 ) skeletal muscle, ( 7 ) kidney, and ( 8 ) testis. ( B ) Northern blot analysis of the expression of iap -2 in testes. A rat multiple tissue Northern blot (Clontech; same as in (A)) was hybridized sequentially with [ 32 P] dCTP labeled DNA probes specific to iap -2 coding region, BIR domain and the RING zing finger. The blots were processed as in (A). The position of testis-specific transcript is indicated by an asterisk ( * ).
    Figure Legend Snippet: ( A ) Northern blot analysis of rat iap mRNA expression in adult rat tissues. A rat multiple tissue northern blot (Clontech) containing 2 μg/lane poly(A) + RNA per lane was probed sequentially with [ 32 P] dCTP (Amersham) labeled, random primed (Amersham Rediprime) DNA probes derived from the coding regions of rat iap-1 , iap-2 , iap-3, and β-actin (control). Blots were hybridized overnight in 5 × SSPE/10 × Denhardt's solution/100 μg/ml salmon sperm DNA/ 50% formamide/ 2% SDS and then washed with 0.2 × SSC/ 0.1% SDS at 50°C. The position and sizes of the markers are indicated on the left. The tissues represented on the blot are as follows: ( 1 ) heart, ( 2 ) brain, ( 3 ) spleen, ( 4 ) lung, ( 5 ) liver, ( 6 ) skeletal muscle, ( 7 ) kidney, and ( 8 ) testis. ( B ) Northern blot analysis of the expression of iap -2 in testes. A rat multiple tissue Northern blot (Clontech; same as in (A)) was hybridized sequentially with [ 32 P] dCTP labeled DNA probes specific to iap -2 coding region, BIR domain and the RING zing finger. The blots were processed as in (A). The position of testis-specific transcript is indicated by an asterisk ( * ).

    Techniques Used: Northern Blot, Expressing, Labeling, Random Primed, Derivative Assay

    The relative expression levels of the rat iap genes in various tissues as detected by Northern or Western blot analysis.
    Figure Legend Snippet: The relative expression levels of the rat iap genes in various tissues as detected by Northern or Western blot analysis.

    Techniques Used: Expressing, Northern Blot, Western Blot

    Related Articles

    Northern Blot:

    Article Title: Isolation of two isoforms of phosphodiesterase 5 from rat penis.
    Article Snippet: .. Rat multiple tissue Northern blots were purchased from Clontech Inc. (Palo Alto, CA, USA). ..

    Article Title: Method of preparing GALR2 receptors composition
    Article Snippet: Northern Blots Human brain multiple tissue northern blots (MTN blots II and III, Clontech, Palo Alto, Calif.) carrying mRNA purified from various human brain areas may be hybridized according to the manufacturers' specifications. .. Rat multiple tissue northern blots including multiple brain tissue blots (rat MTN blot, Clontech, Palo Alto, Calif.) carrying mRNA purified from various rat tissues also may be hybridized at high stringency according to the manufacturer's specifications. ..

    Article Title: An Open Rectifier Potassium Channel with Two Pore Domains in Tandem Cloned from Rat Cerebellum
    Article Snippet: .. Copyright © 1998 Society for Neuroscience 0270-6474/98/180868-10$05.00/0 The Journal of Neuroscience, February 1, 1998, 18(3):868–877 Grand Island, NY) with [a-32P]dCTP (Amersham, Arlington Heights, IL) included in the reaction mixture to produce a labeled probe for hybridization against commercially available human brain and rat multiple tissue Northern blots (Clontech, Palo Alto, CA) and an additional blot of rat cerebellar RNA alone. ..

    Article Title: Molecular cloning of a novel form (two-repeat) protein related to voltage-gated sodium and calcium channels.
    Article Snippet: t s a W A t ( c Voltage-gated Ca and Na channels share similar tructure: four homologous domains (I–IV), each with ix transmembrane segments (S1–S6).. They may be ormed by two rounds of duplication of a single chanel domain similar to voltage-activated potassium hannels.. However, the channels with the intermedite structure, namely, two-domain channels have not et been identified.

    Article Title: Uses of galanin GALR2 receptors
    Article Snippet: Northern Blots Human brain multiple tissue northern blots (MTN blots II and III, Clontech, Palo Alto, Calif.) carrying mRNA purified from various human brain areas may be hybridized according to the manufacturers' specifications. .. Rat multiple tissue northern blots including multiple brain tissue blots (rat MTN blot, Clontech, Palo Alto, Calif.) carrying mRNA purified from various rat tissues also may be hybridized at high stringency according to the manufacturer's specifications. ..

    Article Title: An Open Rectifier Potassium Channel with Two Pore Domains in Tandem Cloned from Rat Cerebellum
    Article Snippet: .. This fragment was randomly primed (RadPrime DNA labeling system; GIBCO BRL, Grand Island, NY) with [α- 32 P]dCTP (Amersham, Arlington Heights, IL) included in the reaction mixture to produce a labeled probe for hybridization against commercially available human brain and rat multiple tissue Northern blots (Clontech, Palo Alto, CA) and an additional blot of rat cerebellar RNA alone. ..

    Article Title: Three Novel Proteins of the Syntaxin/SNAP-25 Family
    Article Snippet: .. Human and rat multiple tissue Northern blots were purchased from CLONTECH. ..

    Purification:

    Article Title: Method of preparing GALR2 receptors composition
    Article Snippet: Northern Blots Human brain multiple tissue northern blots (MTN blots II and III, Clontech, Palo Alto, Calif.) carrying mRNA purified from various human brain areas may be hybridized according to the manufacturers' specifications. .. Rat multiple tissue northern blots including multiple brain tissue blots (rat MTN blot, Clontech, Palo Alto, Calif.) carrying mRNA purified from various rat tissues also may be hybridized at high stringency according to the manufacturer's specifications. ..

    Article Title: Uses of galanin GALR2 receptors
    Article Snippet: Northern Blots Human brain multiple tissue northern blots (MTN blots II and III, Clontech, Palo Alto, Calif.) carrying mRNA purified from various human brain areas may be hybridized according to the manufacturers' specifications. .. Rat multiple tissue northern blots including multiple brain tissue blots (rat MTN blot, Clontech, Palo Alto, Calif.) carrying mRNA purified from various rat tissues also may be hybridized at high stringency according to the manufacturer's specifications. ..

    Labeling:

    Article Title: An Open Rectifier Potassium Channel with Two Pore Domains in Tandem Cloned from Rat Cerebellum
    Article Snippet: .. Copyright © 1998 Society for Neuroscience 0270-6474/98/180868-10$05.00/0 The Journal of Neuroscience, February 1, 1998, 18(3):868–877 Grand Island, NY) with [a-32P]dCTP (Amersham, Arlington Heights, IL) included in the reaction mixture to produce a labeled probe for hybridization against commercially available human brain and rat multiple tissue Northern blots (Clontech, Palo Alto, CA) and an additional blot of rat cerebellar RNA alone. ..

    Article Title: An Open Rectifier Potassium Channel with Two Pore Domains in Tandem Cloned from Rat Cerebellum
    Article Snippet: .. This fragment was randomly primed (RadPrime DNA labeling system; GIBCO BRL, Grand Island, NY) with [α- 32 P]dCTP (Amersham, Arlington Heights, IL) included in the reaction mixture to produce a labeled probe for hybridization against commercially available human brain and rat multiple tissue Northern blots (Clontech, Palo Alto, CA) and an additional blot of rat cerebellar RNA alone. ..

    Hybridization:

    Article Title: An Open Rectifier Potassium Channel with Two Pore Domains in Tandem Cloned from Rat Cerebellum
    Article Snippet: .. Copyright © 1998 Society for Neuroscience 0270-6474/98/180868-10$05.00/0 The Journal of Neuroscience, February 1, 1998, 18(3):868–877 Grand Island, NY) with [a-32P]dCTP (Amersham, Arlington Heights, IL) included in the reaction mixture to produce a labeled probe for hybridization against commercially available human brain and rat multiple tissue Northern blots (Clontech, Palo Alto, CA) and an additional blot of rat cerebellar RNA alone. ..

    Article Title: An Open Rectifier Potassium Channel with Two Pore Domains in Tandem Cloned from Rat Cerebellum
    Article Snippet: .. This fragment was randomly primed (RadPrime DNA labeling system; GIBCO BRL, Grand Island, NY) with [α- 32 P]dCTP (Amersham, Arlington Heights, IL) included in the reaction mixture to produce a labeled probe for hybridization against commercially available human brain and rat multiple tissue Northern blots (Clontech, Palo Alto, CA) and an additional blot of rat cerebellar RNA alone. ..

    Expressing:

    Article Title: Molecular cloning of a novel form (two-repeat) protein related to voltage-gated sodium and calcium channels.
    Article Snippet: t s a W A t ( c Voltage-gated Ca and Na channels share similar tructure: four homologous domains (I–IV), each with ix transmembrane segments (S1–S6).. They may be ormed by two rounds of duplication of a single chanel domain similar to voltage-activated potassium hannels.. However, the channels with the intermedite structure, namely, two-domain channels have not et been identified.

    DNA Labeling:

    Article Title: An Open Rectifier Potassium Channel with Two Pore Domains in Tandem Cloned from Rat Cerebellum
    Article Snippet: .. This fragment was randomly primed (RadPrime DNA labeling system; GIBCO BRL, Grand Island, NY) with [α- 32 P]dCTP (Amersham, Arlington Heights, IL) included in the reaction mixture to produce a labeled probe for hybridization against commercially available human brain and rat multiple tissue Northern blots (Clontech, Palo Alto, CA) and an additional blot of rat cerebellar RNA alone. ..



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    rat multiple tissue northern blot  (TaKaRa)
    90
    TaKaRa rat multiple tissue northern blot
    ( A ) <t>Northern</t> <t>blot</t> analysis of <t>rat</t> iap mRNA expression in adult rat tissues. A rat <t>multiple</t> <t>tissue</t> northern blot (Clontech) containing 2 μg/lane poly(A) + RNA per lane was probed sequentially with [ 32 P] dCTP (Amersham) labeled, random primed (Amersham Rediprime) DNA probes derived from the coding regions of rat iap-1 , iap-2 , iap-3, and β-actin (control). Blots were hybridized overnight in 5 × SSPE/10 × Denhardt's solution/100 μg/ml salmon sperm DNA/ 50% formamide/ 2% SDS and then washed with 0.2 × SSC/ 0.1% SDS at 50°C. The position and sizes of the markers are indicated on the left. The tissues represented on the blot are as follows: ( 1 ) heart, ( 2 ) brain, ( 3 ) spleen, ( 4 ) lung, ( 5 ) liver, ( 6 ) skeletal muscle, ( 7 ) kidney, and ( 8 ) testis. ( B ) Northern blot analysis of the expression of iap -2 in testes. A rat multiple tissue Northern blot (Clontech; same as in (A)) was hybridized sequentially with [ 32 P] dCTP labeled DNA probes specific to iap -2 coding region, BIR domain and the RING zing finger. The blots were processed as in (A). The position of testis-specific transcript is indicated by an asterisk ( * ).
    Rat Multiple Tissue Northern Blot, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+multiple+tissue+northern+blots/Rat+Brain+Poly+A%2B+RNA/pmc00065541-85-1-6
    Average 90 stars, based on 1 article reviews
    rat multiple tissue northern blot - by Bioz Stars, 2026-09
    90/100 stars
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    rat multiple-tissue northern blot  (OriGene)
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    OriGene rat multiple-tissue northern blot
    ( A ) <t>Northern</t> <t>blot</t> analysis of <t>rat</t> iap mRNA expression in adult rat tissues. A rat <t>multiple</t> <t>tissue</t> northern blot (Clontech) containing 2 μg/lane poly(A) + RNA per lane was probed sequentially with [ 32 P] dCTP (Amersham) labeled, random primed (Amersham Rediprime) DNA probes derived from the coding regions of rat iap-1 , iap-2 , iap-3, and β-actin (control). Blots were hybridized overnight in 5 × SSPE/10 × Denhardt's solution/100 μg/ml salmon sperm DNA/ 50% formamide/ 2% SDS and then washed with 0.2 × SSC/ 0.1% SDS at 50°C. The position and sizes of the markers are indicated on the left. The tissues represented on the blot are as follows: ( 1 ) heart, ( 2 ) brain, ( 3 ) spleen, ( 4 ) lung, ( 5 ) liver, ( 6 ) skeletal muscle, ( 7 ) kidney, and ( 8 ) testis. ( B ) Northern blot analysis of the expression of iap -2 in testes. A rat multiple tissue Northern blot (Clontech; same as in (A)) was hybridized sequentially with [ 32 P] dCTP labeled DNA probes specific to iap -2 coding region, BIR domain and the RING zing finger. The blots were processed as in (A). The position of testis-specific transcript is indicated by an asterisk ( * ).
    Rat Multiple Tissue Northern Blot, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+multiple+tissue+northern+blots/rat+multiple+tissue+northern+blots/pmc03873836-89-3-7
    Average 90 stars, based on 1 article reviews
    rat multiple-tissue northern blot - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Northern blot analysis of rat iap mRNA expression in adult rat tissues. A rat multiple tissue northern blot (Clontech) containing 2 μg/lane poly(A) + RNA per lane was probed sequentially with [ 32 P] dCTP (Amersham) labeled, random primed (Amersham Rediprime) DNA probes derived from the coding regions of rat iap-1 , iap-2 , iap-3, and β-actin (control). Blots were hybridized overnight in 5 × SSPE/10 × Denhardt's solution/100 μg/ml salmon sperm DNA/ 50% formamide/ 2% SDS and then washed with 0.2 × SSC/ 0.1% SDS at 50°C. The position and sizes of the markers are indicated on the left. The tissues represented on the blot are as follows: ( 1 ) heart, ( 2 ) brain, ( 3 ) spleen, ( 4 ) lung, ( 5 ) liver, ( 6 ) skeletal muscle, ( 7 ) kidney, and ( 8 ) testis. ( B ) Northern blot analysis of the expression of iap -2 in testes. A rat multiple tissue Northern blot (Clontech; same as in (A)) was hybridized sequentially with [ 32 P] dCTP labeled DNA probes specific to iap -2 coding region, BIR domain and the RING zing finger. The blots were processed as in (A). The position of testis-specific transcript is indicated by an asterisk ( * ).

    Journal: BMC Genomics

    Article Title: Cloning and characterization of the rat homologues of the Inhibitor of Apoptosis protein 1, 2, and 3 genes.

    doi: 10.1186/1471-2164-3-5

    Figure Lengend Snippet: ( A ) Northern blot analysis of rat iap mRNA expression in adult rat tissues. A rat multiple tissue northern blot (Clontech) containing 2 μg/lane poly(A) + RNA per lane was probed sequentially with [ 32 P] dCTP (Amersham) labeled, random primed (Amersham Rediprime) DNA probes derived from the coding regions of rat iap-1 , iap-2 , iap-3, and β-actin (control). Blots were hybridized overnight in 5 × SSPE/10 × Denhardt's solution/100 μg/ml salmon sperm DNA/ 50% formamide/ 2% SDS and then washed with 0.2 × SSC/ 0.1% SDS at 50°C. The position and sizes of the markers are indicated on the left. The tissues represented on the blot are as follows: ( 1 ) heart, ( 2 ) brain, ( 3 ) spleen, ( 4 ) lung, ( 5 ) liver, ( 6 ) skeletal muscle, ( 7 ) kidney, and ( 8 ) testis. ( B ) Northern blot analysis of the expression of iap -2 in testes. A rat multiple tissue Northern blot (Clontech; same as in (A)) was hybridized sequentially with [ 32 P] dCTP labeled DNA probes specific to iap -2 coding region, BIR domain and the RING zing finger. The blots were processed as in (A). The position of testis-specific transcript is indicated by an asterisk ( * ).

    Article Snippet: A rat multiple tissue northern blot (Clontech) containing 2 μg/lane poly(A) + RNA per lane was probed sequentially with [ 32 P] dCTP (Amersham) labeled, random primed (Amersham Rediprime) DNA probes derived from the coding regions of rat iap-1 , iap-2 , and iap-3, or β-actin, respectively .

    Techniques: Northern Blot, Expressing, Labeling, Random Primed, Derivative Assay

    The relative expression levels of the rat iap genes in various tissues as detected by Northern or Western blot analysis.

    Journal: BMC Genomics

    Article Title: Cloning and characterization of the rat homologues of the Inhibitor of Apoptosis protein 1, 2, and 3 genes.

    doi: 10.1186/1471-2164-3-5

    Figure Lengend Snippet: The relative expression levels of the rat iap genes in various tissues as detected by Northern or Western blot analysis.

    Article Snippet: A rat multiple tissue northern blot (Clontech) containing 2 μg/lane poly(A) + RNA per lane was probed sequentially with [ 32 P] dCTP (Amersham) labeled, random primed (Amersham Rediprime) DNA probes derived from the coding regions of rat iap-1 , iap-2 , and iap-3, or β-actin, respectively .

    Techniques: Expressing, Northern Blot, Western Blot